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16S Ribosomal RNA Gene Sequencing
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A research team is conducting a study on bacterial diversity in the human gut. They decide to use 16S ribosomal RNA gene sequencing to identify the bacterial species present. Which of the following statements best explains why this method is advantageous for classifying these bacteria?

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A

It can identify all types of microorganisms, including viruses and fungi.

B

It targets a highly conserved region of the bacterial genome, allowing for effective comparison across diverse species.

C

It requires the isolation of pure bacterial cultures before sequencing can be performed.

D

It can only identify bacteria that have been previously studied and cataloged.

Understanding the Answer

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The 16S rRNA gene contains parts that stay almost the same in all bacteria, called conserved regions. Other options are incorrect because The 16S rRNA gene is only found in bacteria, not in viruses or fungi; The method can work directly on mixed DNA from a sample, so pure cultures are not needed.

Key Concepts

Bacterial classification
16S ribosomal RNA gene sequencing
Microbial diversity
Topic

16S Ribosomal RNA Gene Sequencing

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medium level question

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Definition
Definition

16S ribosomal RNA (rRNA) gene sequencing is a genomic approach used to classify bacteria based on the highly conserved 16S rRNA gene. This technique allows for the profiling and taxonomic classification of bacterial species present in complex microbial populations.

Topic Definition

16S ribosomal RNA (rRNA) gene sequencing is a genomic approach used to classify bacteria based on the highly conserved 16S rRNA gene. This technique allows for the profiling and taxonomic classification of bacterial species present in complex microbial populations.

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