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HomeHomework Helpmicrobiology16S rRNA Gene Sequencing

16S rRNA Gene Sequencing

16S ribosomal RNA (rRNA) gene sequencing is a genomic approach used to classify bacteria based on the highly conserved 16S rRNA gene. This technique allows for the profiling and taxonomic classification of bacterial species present in complex microbial populations.

intermediate
5 hours
Microbiology
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Overview

16S ribosomal RNA gene sequencing is a powerful tool in microbiology that allows scientists to identify and classify bacteria based on their genetic material. By analyzing the sequences of the 16S rRNA gene, researchers can uncover the diversity of microbial life and understand evolutionary relation...

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Key Terms

rRNA
Ribosomal RNA, a type of RNA that forms part of the ribosome.

Example: rRNA is essential for protein synthesis in all living cells.

PCR
Polymerase Chain Reaction, a technique used to amplify DNA sequences.

Example: PCR is used to create millions of copies of a specific DNA segment.

Phylogenetics
The study of evolutionary relationships among biological entities.

Example: Phylogenetics helps scientists understand how species are related.

Bioinformatics
The application of computer technology to manage biological information.

Example: Bioinformatics tools are used to analyze DNA sequences.

Microbiome
The collection of microorganisms living in a particular environment, including the human body.

Example: The gut microbiome plays a crucial role in digestion and health.

Next-Generation Sequencing
A modern sequencing technology that allows for rapid sequencing of large amounts of DNA.

Example: Next-generation sequencing can analyze entire genomes quickly.

Related Topics

Metagenomics
The study of genetic material recovered directly from environmental samples.
advanced
Microbial Ecology
The study of the interactions between microorganisms and their environment.
intermediate
Genomic Sequencing
The process of determining the complete DNA sequence of an organism's genome.
advanced

Key Concepts

Bacterial IdentificationPhylogeneticsMicrobial DiversityGenomic Analysis