Overview
Standard curve generation is a fundamental technique in analytical chemistry that allows scientists to determine the concentration of unknown samples by comparing them to known standards. By preparing standard solutions and measuring their absorbance, researchers can create a graph that illustrates ...
Key Terms
Example: A standard curve for glucose concentration plotted against absorbance.
Example: Calibrating a spectrophotometer before use.
Example: A 1M solution of sodium chloride.
Example: An absorbance reading of 0.5 at 600 nm.
Example: Using a spectrophotometer to measure the absorbance of a dye solution.
Example: Using linear regression to find the best fit line for a standard curve.
Related Topics
Spectrophotometry
The study of how light interacts with matter, particularly in measuring absorbance.
intermediateQuantitative Analysis
The determination of the quantity of a substance in a sample using various analytical techniques.
advancedAnalytical Chemistry
The branch of chemistry focused on the qualitative and quantitative analysis of substances.
advancedKey Concepts
CalibrationConcentrationAbsorbanceLinear Regression