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HomeHomework HelpbiologyGene Cloning

Gene Cloning

Gene cloning is the process of creating identical copies of a specific gene or DNA segment by isolating it from an organism and inserting it into a vector, which is then introduced into a host cell for replication. This technique is commonly used in genetic research, biotechnology, and medicine to study gene function and produce proteins.

intermediate
3 hours
Biology
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Overview

Gene cloning is a fundamental technique in molecular biology that allows scientists to create copies of specific genes. This process involves several steps, including the use of restriction enzymes to cut DNA, the incorporation of the gene into a vector, and the transformation of host cells to produ...

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Key Terms

DNA
Deoxyribonucleic acid, the molecule that carries genetic information.

Example: DNA is found in the nucleus of cells.

Gene
A segment of DNA that contains instructions for making a specific protein.

Example: The BRCA1 gene is linked to breast cancer.

Restriction Enzyme
An enzyme that cuts DNA at specific sequences.

Example: EcoRI is a commonly used restriction enzyme.

Vector
A DNA molecule used to deliver genetic material into a host cell.

Example: Plasmids are often used as vectors in gene cloning.

Transformation
The process of introducing foreign DNA into a host cell.

Example: Bacterial transformation is a common method in gene cloning.

Clone
A genetically identical copy of a gene or organism.

Example: Scientists can create clones of specific genes for research.

Related Topics

CRISPR Technology
A revolutionary gene-editing tool that allows for precise modifications to DNA.
advanced
Genetic Engineering
The direct manipulation of an organism's genes using biotechnology.
intermediate
Synthetic Biology
An interdisciplinary field that combines biology and engineering to design and construct new biological parts.
advanced

Key Concepts

DNA replicationRestriction enzymesVectorTransformation