Overview
Southern blotting is a powerful technique in molecular biology that allows scientists to identify specific DNA sequences within a complex mixture. By combining gel electrophoresis and hybridization, researchers can separate DNA fragments and use labeled probes to detect sequences of interest. This m...
Key Terms
Example: DNA is found in the nucleus of cells.
Example: Gel electrophoresis is used to analyze PCR products.
Example: DNA hybridization is used in Southern blotting.
Example: A probe can be used to find a gene of interest.
Example: Membrane transfer is a key step in Southern blotting.
Example: Nucleic acids store and transmit genetic information.
Related Topics
Northern Blotting
A technique similar to Southern blotting but used for detecting RNA sequences.
intermediateWestern Blotting
A method for detecting specific proteins in a sample using antibodies.
intermediatePCR (Polymerase Chain Reaction)
A technique used to amplify specific DNA sequences, often used before Southern blotting.
intermediateKey Concepts
DNA detectionGel electrophoresisHybridizationProbe