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HomeHomework HelpmicrobiologyGram-positive vs Gram-negative

Gram-positive vs Gram-negative

Gram-positive bacteria are characterized by a thick peptidoglycan layer in their cell wall, which retains the crystal violet stain used in the Gram staining procedure, resulting in a purple appearance. In contrast, Gram-negative bacteria have a thinner peptidoglycan layer and an outer membrane, which does not retain the crystal violet stain but takes up the counterstain, leading to a pink appearance.

intermediate
2 hours
Microbiology
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Gram-positive and Gram-negative bacteria are classified based on their cell wall structure, which significantly influences their staining properties and susceptibility to antibiotics. Gram-positive bacteria have a thick peptidoglycan layer that retains the crystal violet dye during Gram staining, ap...

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Key Terms

Peptidoglycan
A polymer that makes up the cell wall of bacteria, providing structural support.

Example: Gram-positive bacteria have a thick layer of peptidoglycan.

Crystal Violet
A dye used in the Gram staining process to color bacteria.

Example: Crystal violet stains Gram-positive bacteria purple.

Safranin
A counterstain used in Gram staining that colors Gram-negative bacteria pink.

Example: After safranin application, Gram-negative bacteria appear pink.

Outer Membrane
A lipid bilayer found in Gram-negative bacteria that protects against certain antibiotics.

Example: The outer membrane makes Gram-negative bacteria more resistant.

Pathogen
An organism that causes disease.

Example: Some Gram-positive bacteria are known pathogens.

Antibiotic
A substance that kills or inhibits the growth of bacteria.

Example: Penicillin is an antibiotic effective against Gram-positive bacteria.

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Key Concepts

Cell wall structureGram stainingAntibiotic resistancePathogenicity