Overview
Gel electrophoresis is a fundamental technique in molecular biology that allows scientists to separate and analyze nucleic acids and proteins. By applying an electric field to a gel matrix, molecules migrate based on their size and charge, forming distinct bands that can be visualized and interprete...
Key Terms
Example: Agarose gels are commonly used for DNA separation.
Example: Polyacrylamide gels provide higher resolution for protein separation.
Example: Electrodes are placed in the electrophoresis chamber to create an electric field.
Example: TBE buffer is often used in DNA electrophoresis.
Example: Ethidium bromide is a common stain for DNA visualization.
Example: Smaller DNA fragments migrate faster than larger ones.
Related Topics
PCR (Polymerase Chain Reaction)
A technique used to amplify DNA segments, making them easier to analyze.
intermediateWestern Blotting
A method used to detect specific proteins in a sample after gel electrophoresis.
advancedDNA Sequencing
The process of determining the exact sequence of nucleotides in a DNA molecule.
advancedKey Concepts
Separation of moleculesGel matrixElectric fieldVisualization of results