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HomeHomework HelpbiologyDNA Sequencing

DNA Sequencing

DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule. This technique allows for the analysis of genetic information, enabling researchers to study genes, genetic variation, and the functions of different DNA sequences.

intermediate
3 hours
Biology
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Overview

DNA sequencing is a powerful tool that allows scientists to read the genetic code of organisms. By determining the order of nucleotides, researchers can identify genetic variations that contribute to diseases, understand evolutionary relationships, and develop new medical treatments. The advancement...

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Key Terms

Nucleotide
The basic building block of DNA, consisting of a sugar, phosphate group, and a nitrogenous base.

Example: A nucleotide can be adenine, thymine, cytosine, or guanine.

Sanger Sequencing
A method of DNA sequencing based on selective incorporation of chain-terminating dideoxynucleotides.

Example: Sanger sequencing is often used for small-scale projects.

Next-Generation Sequencing (NGS)
A high-throughput method that allows for rapid sequencing of large amounts of DNA.

Example: NGS can sequence an entire genome in a single run.

Genome
The complete set of genes or genetic material present in a cell or organism.

Example: The human genome contains approximately 3 billion base pairs.

Bioinformatics
The application of computer technology to manage biological information.

Example: Bioinformatics tools are used to analyze DNA sequencing data.

Allele
Different forms of a gene that can exist at a specific locus on a chromosome.

Example: An individual may have two different alleles for a gene.

Related Topics

Genetic Engineering
The manipulation of an organism's genes using biotechnology.
advanced
CRISPR Technology
A revolutionary tool for editing genes with precision.
advanced
Molecular Cloning
The process of making multiple copies of a specific DNA sequence.
intermediate

Key Concepts

NucleotidesSequencing TechniquesApplications in MedicineGenetic Variation