Overview
Western blotting is a powerful technique used in molecular biology to detect specific proteins in a sample. It involves several steps, including gel electrophoresis to separate proteins, transferring them to a membrane, and using antibodies to identify the target proteins. This method is widely used...
Key Terms
Example: Monoclonal antibodies are used in Western blotting to detect specific proteins.
Example: The target protein in Western blotting acts as an antigen.
Example: Proteins are loaded into a gel and separated during electrophoresis.
Example: After gel electrophoresis, proteins are transferred to a nitrocellulose membrane.
Example: Chemiluminescent detection allows for sensitive visualization of protein bands.
Example: A protein ladder helps estimate the size of separated proteins.